Subtilisin

Synonyms: Subtilisin Carlsberg, Subtilopeptidase A, Bacterial Alkaline Protease

CAS #: 9014-01-1

EC #: 3.4.21.62

Sigma's Subtilisins are produced from fermentation of Bacillus licheniformis. We offer several grades and formulations including high purity lyophilized powders as well as economical industrial grade solutions.

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Physical properties and Specificity

MW: Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and a of MW 27 KDa.2

Specificity: Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Isoelectric point: pI = 9.4

E1%(280nm) = 8.6

Sedimentation coefficient: S20,w = 2.773 <>

Activity Determination and Optimal Conditions

Unit definition: One Sigma Unit will hydrolyze casein to produce color equivalent to 1.0 mmole (181 µg) of tyrosine per minute at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).