7-TD-1

Cat.No.

CSC-C1056

Description

Murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6

Organism

Mouse

Karyotype

Murine karyotype

Source

B cell hybridoma

Culture Properties

suspension

Morphology

round cells, growing single or clustered in suspension

Recommended Medium

15% + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell di

https://www.creative-bioarray.com/7-TD-1-CSC-C1056-item-1524.htm