Description
Established from an X-irradiated C3H/HeNSIc mouse; cells were described to be stroma-independent, responsive to erythropoietin and to produce hemoglobin (augmented by IL-3)
Culture Properties
suspension
Morphology
large, round, single cells in suspension
Recommended Medium
90% SuperCult® AlphaMEM
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
https://www.creative-bioarray.com/ELM-I-1-CSC-C1060-item-1528.htm