Description
Established in vitro from the AS-30-D tumor ascites (by CLS Cell Lines Service), RAP-Test negative. The species was confirmed by Real-Time PCR.
Karyotype
Hypodiploid rat karyotype with 12% tetraploidy; 38 (35-41).
Culture Properties
suspension
Recommended Medium
RPMI 1640 medium supplemented with 4.5g/L glucose, 2 mM L-glutamine
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
https://www.creative-bioarray.com/AS-30D-A-S-30D-CSC-C1079-item-1544.htm