Description
Derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and
decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for
Biological Safety (ZKBS)
Karyotype
Murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells)
Source
NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line
Culture Properties
monolayer
Morphology
cells grow as adherent fibroblastic monolayers
Recommended Medium
90% DMEM
https://www.creative-bioarray.com/OMEGA-E-CSC-C1083-item-1547.htm