Description
Established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats
Karyotype
Rat hypertriploid karyotype with 6% polyploidy - 67(67-72)<3n> - corresponds to published karyotype
Source
glomerular endothelial cells
Culture Properties
monolayer
Morphology
adherent epitheloid cells growing as monolayer (after confluency cells tend to grow in multilayers)
Recommended Medium
90% RPMI-1640
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
https://www.creative-bioarray.com/RGE-CSC-C1084-item-1548.htm