Description
Established from the bone marrow of a C3H/HeJ mouse
Karyotype
Murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent
Morphology
round cells growing in aggregates, mostly loosely adherent, some cells have long processes
Recommended Medium
80%RPMI-1640 + 1 mM sodium pyruvate + 1 mM non-essential amino acids
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
https://www.creative-bioarray.com/DMBM-2-CSC-C1085-item-1549.htm