Stability and precautions of acridine ester (NSP-SA-NHS)

Acridine ester NSP-SA-NHS is a highly stable and sensitive chemiluminescence reagent, commonly used for labeling proteins, peptides, antibodies, nucleic acids, etc. And in an alkaline hydrogen peroxide environment, labeling proteins can self emit without the need for enzyme catalysis, making the operation simple.

The main factors affecting the stability of acridine ester NSP-SA-NHS are as follows:

1. It is stable in acidic solutions with a pH value less than 4.8, and can be stored with protein conjugates for about 4 weeks at room temperature. Its light quantum yield remains basically unchanged, and freeze-dried products can be stored for more than a year at minus 20 ℃.

2. When the pH value of the solution is greater than 4.8, acridine ester NSP-SA-NHS and its compounds are prone to hydrolysis, which reduces their stability. That is, the higher the pH value, the higher the degree of hydrolysis, and the stability immediately decreases.

3. The stability of acridine ester NSP-SA-NHS is greatly affected by temperature. The higher the temperature, the higher the degree of hydrolysis, and the lower the stability. The hydrolysis process is a dark reaction process that does not emit light.

matters needing attention:

1. After coupling, try to use an acidic buffer solution with a pH value less than 4.8 for storage, preferably in a sealed low-temperature environment. If conditions permit, the sample can be made into freeze-dried products for storage.

2. The luminescence process of acridine ester NSP-SA-NHS is very short, and internal photometers and photon detectors are needed to capture key information during relevant experiments or detection to ensure the accuracy of experimental and detection results.

3. Acridine ester NSP-SA-NHS is prone to partial decomposition under the influence of light, which affects the luminescence effect. Therefore, it is advisable to avoid light environment during relevant experimental operations or storage.

4. The stability of acridine ester NSP-SA-NHS is greatly affected by alkaline and oxidative environments, so it is necessary to prepare corresponding non alkaline and non oxidative systems for experiments based on the stability of the labeled substance, in order to obtain more accurate results.

In addition to the acridine ester NSP-SA-NHS, the chemiluminescence reagents produced by Desheng also include products such as luminol, isoluminol, and luminol monosodium salt. The product quality is high, the production process is stable, and the batch differences are small. There is a large amount of stock available and the delivery speed is fast. If you need it, please contact us now!