Acridinium salt NSP-SA-NHS CAS199293-83-9

Acridine ester NSP-SA-NHS is a highly regarded chemiluminescence reagent, which is not only favored by customers, but also has excellent performance. At present, most manufacturers produce acridine ester NSP-SA-NHS in the form of yellow powder for the convenience of transportation and storage. When used, it is formulated into a solution and can emit light without enzyme catalysis after contact with hydrogen peroxide. It is suitable for various luminescence analysis experiments. What are its specific luminescent characteristics? Below is a specific introduction for everyone.

1. Luminescence wavelength: Under the attack of hydrogen peroxide ions, it can generate unstable ethylene oxide, which then decomposes into carbon dioxide and excited form of acridone. When it returns to the ground state, it can emit photons with an absorption wavelength of 430nm. This wavelength makes the luminescence process rapid, typically ending in 2 seconds, achieving the goal of rapid luminescence while also being absorbed and emitted by most biomolecules.

2. Luminous intensity: Its luminous intensity generally varies depending on the concentration and pH value of the test sample. If the environmental conditions of the test sample are the same, the intensity can reach its peak in 0.4 seconds after adding the excitation solution, and the half-life is around 0.9 seconds.

3. Stability: Acridine ester NSP-SA-NHS has good stability and will not lose its luminescent properties after being sealed and stored at room temperature in the dark for a period of time. However, it is also important to pay attention to the surrounding storage temperature. It is recommended to store it in a refrigerator and check the expiration date of the reagents before use, and prepare and use them in a timely manner.

4. React sensitive: It has high sensitivity and does not require catalysts or enhancers in experimental reactions. It can emit light by simply experimenting in a dilute alkaline solution with H2O2. Its structure is simple and easy to undergo chemical reactions.

5. Wide application range: It is suitable for labeling various biomolecules, such as proteins, nucleic acids, sugars, etc. Due to its sensitivity and stability, it has also been applied in research such as fluorescent probes, receptor analysis, and peptide detection.

The luminescent characteristics of acridine ester represent its quality and also indicate the effectiveness of the experiment, so it is important to ensure that the purchased product is problem free before use. Desheng is a professional supplier of luminescent reagents. The acridine ester series products are of analytical grade, with pure appearance and color, uniform powder, and easy preparation and operation. It is one of the manufacturers that most people are willing to choose. If you also have relevant intentions, please click on the website to inquire about details!