In the fields of biochemistry and medical diagnosis, chromogenic substrates are an indispensable part of experimental detection. Among them, ALPS (N-ethyl-N - (3-sulfonylpropyl) aniline sodium salt) has attracted much attention as a novel Trinder's reagent chromogenic substrate due to its chemical properties and wide application prospects. This article will explore in detail the application of ALPS in biochemical detection and analyze its advantages and disadvantages, in order to provide reference for research and practice in related fields.
Application of ALPS in Biochemical Testing
ALPS, as a highly sensitive chromogenic substrate, plays an important role in biochemical detection. It can undergo oxidative coupling reaction with hydrogen peroxide and peroxidase to generate stable color products. This characteristic gives ALPS significant advantages in measuring hydrogen peroxide activity, enzyme activity, and substrate concentration. For example, when measuring certain trace test items in serum, ALPS can accurately and quickly provide detection results, providing strong support for medical diagnosis.
Advantages of ALPS
1. High sensitivity: ALPS has a high molar absorbance, which can perform well in the determination of trace analytes, improving the sensitivity and accuracy of detection.
2. Good water solubility: ALPS has good water solubility and is easy to evenly distribute in solution, which helps to achieve rapid and uniform biochemical reactions.
3. Good stability: Compared with other chromogenic substrates, ALPS exhibits good stability in solution and test line detection systems, and can maintain the color of the color product for a long time, reducing the occurrence of errors.
4. Obvious color reaction: ALPS's color products are brightly colored, easy to observe and compare, which helps to improve the intuitiveness and accuracy of the detection results.
【English name】N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt 【Chinese name】N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt 【Abbreviation】ADPS 【CAS#】82611-88-9 【Molecular weight】295.33 【Molecular formula】C12H18NO4SNa 【Storage ...
【English name】N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline sodium salt dihydrate 【Chinese name】N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline sodium salt dihydrate 【Abbreviation】ADOS 【CAS#】82692-96-4 【Molecular weight】347.36035 【Molecular ...
【English name】N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 【Chinese name】N-ethyl-N - (2-hydroxy-3-sulfopropyl) - 3-methylaniline sodium salt dihydrate 【Abbreviation】TOOS 【CAS#】82692-93-1 【Molecular weight】295.33 【Molecular formula】C12H18NNaO4S ...