DAOS N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt

DAOS, as a sodium sulfonate derivative containing aniline groups, has a wide range of applications in the field of biochemical detection. Its color development principle is based on specific chemical reactions, quantifying the content of the target substance by measuring the absorbance of the reaction product. This article will explore in detail the color development principle of DAOS and introduce its application in biochemical detection.

DAOS color development principle

The color development principle of DAOS is mainly based on its use as a chromogenic substrate in Trinder's reagent, which undergoes oxidation reaction with hydrogen peroxide (H2O2) under the catalysis of peroxidase (POD). This reaction causes a structural change in DAOS, resulting in a color change. The specific process is as follows:

DAOS undergoes an oxidation reaction with hydrogen peroxide under the action of peroxidase. During this process, the aniline group of DAOS is oxidized, generating corresponding oxidation products. This oxidation product has a specific absorption wavelength, allowing us to quantify the degree of reaction by measuring its absorbance.

Secondly, the color reaction of DAOS has high specificity and sensitivity. Due to the fact that peroxidase only catalyzes oxidation reactions of specific substrates, the color reaction of DAOS can selectively detect target substances. Meanwhile, the color reaction of DAOS has a wide linear range, allowing us to accurately determine target substances at different concentrations.

In addition, the color reaction of DAOS also has good stability and repeatability. Under appropriate conditions, DAOS can maintain a longer color development time, making it convenient for us to conduct batch testing and long-term observation. Meanwhile, the color reaction of DAOS is less affected by interfering factors, making the detection results more accurate and reliable.

Application of DAOS in Biochemical Testing

DAOS, as an excellent chromogenic substrate, has a wide range of applications in biochemical detection. Here are several typical application cases:

1. High density lipoprotein (HDL) testing

High density lipoprotein is an important lipid component that is closely associated with the risk of cardiovascular disease. By utilizing DAOS as a chromogenic substrate, we can develop highly sensitive and specific HDL detection kits. This kit can quickly and accurately determine the content of HDL in serum or plasma, providing strong support for clinical diagnosis.

2. Blood glucose testing

Blood glucose level is an important index to evaluate the condition of diabetes. DAOS plays a crucial role as a chromogenic substrate in blood glucose detection kits. By synergistic action with enzymes such as glucose oxidase, DAOS can achieve rapid and accurate measurement of blood glucose. This detection method has the advantages of easy operation and reliable results, and is widely used in clinical diagnosis and daily blood glucose monitoring.

3. Triglyceride (TG) detection

Triglycerides are another important component of blood lipids, and their levels are closely related to the risk of cardiovascular disease. By using DAOS as a chromogenic substrate, we can develop a triglyceride detection kit. By measuring the absorbance of reaction products, we can accurately determine the content of triglycerides in serum or plasma, providing important basis for clinical diagnosis.