UB AMC

Ubiquitin AMC is prepared by the C-terminal derivatization of ubiquitin with 7-amino-4-methylcoumarin.

SPECIFICATION OF UB AMC

CAT U1010

Product Name Ub-AMC

Purity ≥95% by HPLC analysis

Appearance Lyophilized powder

Usage Research use only

Storage Shipping at room temperature.

Upon delivery aliquot.

Stored at -20°C or -80°C.

Avoid freeze/thaw cycle.

Introduction Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinating activity where detection sensitivity or continuous monitoring of activity is essential.

APPLICATION OF UB AMC

Can be used for the following applications:

1. Substrate for deubiquitinylating enzyme activity assays.

2. Identification/confirmation of enzyme deubiquitinylation activity.

3. Investigation of deconjugating enzyme-substrate specificity in comparison with alternative UBL substrates.

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