BlueGene Biotech

Bovine Carnitine Palmitoyltransferase I ELISA kit

Metabolism

E11C0055 Bovine Carnitine Palmitoyltransferase I ELISA kit

Bovine Carnitine Palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyltransferase i (CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.

Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit

Product Information

Cat. No. E11C0055

Product Name Bovine Carnitine palmitoyltransferase I ELISA kit

Species Bovine

Product Size 48 Tests / 96 Tests

Concentration 2.5-50 ng/mL

Sensitivity 0.1 ng/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C

Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A (0.5mL) 0 ng/mL 1 vial

STANDARD B (0.5mL) 2.5 ng/mL 1 vial

STANDARD C (0.5mL) 5 ng/mL 1 vial

STANDARD D (0.5mL) 10 ng/mL 1 vial

STANDARD E (0.5mL) 25 ng/mL 1 vial

STANDARD F (0.5mL) 50 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION (100 x) 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.

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