BlueGene Biotech Bovine Catalase ELISA kit

E11C0086 Bovine Catalase ELISA kit

Bovine Catalase ELISA kit is suitable for the detection of samples from Bovine species. Catalase can also be called Cas1, CAT, Cs1.

Specifications of Bovine Catalase ELISA kit

Product Information

Cat. No. E11C0086

Product Name Bovine Catalase ELISA kit

Species Bovine

Product Size 48 Tests / 96 Tests

Concentration 10-250 ng/mL

Sensitivity 1.0 ng/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C

Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A (0.5mL) 0 ng/mL 1 vial

STANDARD B (0.5mL) 10 ng/mL 1 vial

STANDARD C (0.5mL) 25 ng/mL 1 vial

STANDARD D (0.5mL) 50 ng/mL 1 vial

STANDARD E (0.5mL) 100 ng/mL 1 vial

STANDARD F (0.5mL) 250 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION (100 x) 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Catalase ELISA kit

Coefficient of Variance Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery 91-105%

Linearity Diluent Ratio Range %

1:2 87-108

1:4 89-109

1:8 85-111

Specificity/Cross-reactivity No significant cross-reactivity or interference between CAT and analogues was observed.

As one of mouse igg elisa kits manufacturers, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGene Biotech ELISA kits.