BlueGene Biotech Chicken Total Adiponectin ELISA kit

E12A0125 Chicken Total Adiponectin ELISA kit
Chicken Total Adiponectin ELISA kit (a knid of chicken elisa kits )is suitable for the detection of samples from chicken species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.
Product Information
Cat. No. E12A0125
Product Name Chicken Total Adiponectin ELISA kit
Species Chicken
Product Size 48 Tests / 96 Tests
Concentration 1.0-25ng/ml
Sensitivity 0.1 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8°C
Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 1.0 ng/mL 1 vial
STANDARD C (0.5mL) 2.5 ng/mL 1 vial
STANDARD D (0.5mL) 5.0 ng/mL 1 vial
STANDARD E (0.5mL) 10 ng/mL 1 vial
STANDARD F (0.5mL) 25 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial
Principle of the Assay
ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.
Quality Control on Chicken Total Adiponectin ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 87-116.79%
Linearity Diluent Ratio Range %
1:2 96-116
1:4 92-105
1:8 88 -103
Specificity/Cross-reactivity No significant cross-reactivity or interference between ADPN and analogues was observed.
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Bluegene Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of ELISA kits for researchers worldwide since 2008.