The quality of Plant Total RNA Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of total RNA from 25 mg young leaf. Purified RNA could be quantified with spectrophotometer and checked by agarose gel.
The protocol does not require phenol extraction and alcohol precipitation.
4. Finally, the purified total RNA is eluted by RNase-free water.
3. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer.
2. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column.
1. In the process, sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris.
Introduction | |
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Introduction Format: Spin columns Sample: 50 mg plant tissue Operation: Centrifuge / vacuum manifold |