ATP Total RNA Mini Kit (Tissue)

The quality of Plant Total RNA Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of total RNA from 25 mg young leaf. Purified RNA could be quantified with spectrophotometer and checked by agarose gel.



The protocol does not require phenol extraction and alcohol precipitation.

4.  Finally, the purified total RNA is eluted by RNase-free water.

3.  The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer.

2.  In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column.

1.  In the process, sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris.

Introduction
Format: Spin columns
Sample: 50 mg plant tissue
Operation: Centrifuge / vacuum manifold
Operation time: 60 minutes
Yield: 5-30
  • Country:Taiwan
  • telephone:886-886-9687-90671
Introduction
Format: Spin columns
Sample: 50 mg plant tissue
Operation: Centrifuge / vacuum manifold
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ATP Total RNA Mini Kit (Tissue)

The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction. 4. After washing off the contaminants, the purified RNA is eluted by RNase-free water. 3. Then RNA in the chaotropic ...

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ATP Total RNA Maxi Kit

ATP Total RNA Maxi Kit provides a fast and simple method to isolate total RNA from animal tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with ...

Come From ATP Biotech Inc