- Highly specificuantitate only the biologically active mature miRNAs
- Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample
- Significantly reduced sample consumption, particularly when adding the optional preamplification step
- Comprehensive coverage is ideal for human, mouse and rat miRNA profiling
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Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, MegaplexTM Primer Pools provide the flexibility to reach your research goals. Megaplex
Primer Pools provide comprehensive coverage of Sanger miRBase v10, and when used with TaqMan MicroRNA Arrays, offer an ideal microRNA profiling solution.
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- MegaplexTM RT Primers are novel stem-looped RT primer pools that streamline the profiling of hundreds of miRNA targets in a single experiment and reduces the number of RT
reactions and the amount of total RNA required to generate a comprehensive miRNA expression profile
- Optional MegaplexTM PreAmp Primers significantly enhance the ability to detect low expressed miRNAs enabling the generation of a comprehensive expression profile from as
low as 1ng of input total RNA
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MegaplexTM Primer Pools can be used with either individual TaqMan? MicroRNA Assays, or for the ideal profiling workflow, with TaqMan? MicroRNA Arrays. The arrays provide all the
advantages of TaqMan? MicroRNA Assays in a convenient, pre-configured micro fluidic card. The content of each array is matched to the respective Megaplex Primer Pools and contains up to 381
unique TaqMan? MicroRNA Assays, reducing set-up time and experimental variability.
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