The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction.
4. After washing off the contaminants, the purified RNA is eluted by RNase-free water.
3. Then RNA in the chaotropic salt is bonded to the glass fiber matrix of the column.
2. The method uses detergent and a chaotropic salt to lyse cells and inactivate RNase.
1. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube.
Introduction | |
|
Introduction Format: Spin columns Sample: Up to 25 mg of tissue Operation: Centrifuge / vacuum manifold |