The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction.
4. After washing off the contaminants, the purified RNA is eluted by RNase-free water.
3. Then RNA in the chaotropic salt is bonded to the glass fiber matrix of the column.
2. The method uses detergent and a chaotropic salt to lyse cells and inactivate RNase.
1. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube.
Introduction | |
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Introduction Format: Spin columns Sample: Up to 25 mg of tissue Operation: Centrifuge / vacuum manifold |
The quality of Plant Total RNA Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of total RNA from 25 mg young leaf. Purified RNA could be quantified with spectrophotometer and checked by agarose gel. The protocol does not require phenol extraction and ...
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ATP Total RNA Maxi Kit provides a fast and simple method to isolate total RNA from animal tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with ...
Come From ATP Biotech Inc