Synaptogenesis Kit

Figure 2. Drug effects on neuronal health, neurites and synaptogenesis in primary neurons. Different drugs (top panel: zinc, bottom panel: A?1-42 oligomer) were added to the neurons at the indicated concentrations. (A) Mouse cortical neurons at 21 days in vitro (DIV). (B) Rat hippocampal neurons at 50 DIV. Each data point represents six wells in a plate. Neuron and neurite numbers, neurite intensity, neurite length, neurite width, branch point, pre-synaptic marker, post-synaptic marker were measured using the output parameter in Cellomics Neuronal Profiling V3.5 Bioapplication. Values are normalized with the maximum control value and presented as % control. Zinc (treated for 24 hours) and A?1-42 oligomer (treated for 48 hours) decrease neurite count, neurite length and pre- and post-synaptic marker spots as well as synapse count in primary neuronal culture. (Students t-test, *p< 0.05, **p< 0.01, ***p< 0.001)
 
 
 Synaptogenesis Kit
 Product# Price  Product Name  Pack Size  
         
 8408402      -    Synaptogenesis Kit  5 x 96   SELECT   
 8408403      -    Synaptogenesis Kit  50 x 96   SELECT   
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Figure 1. Rat hippocampal neurons were maintained for seven weeks in vitro then stained with the Thermo Scientific Cellomics Synaptogenesis Kit. DAPI staining indicates nuclei. MAP2 stains the cell body and neuritis. PSD95 is a post-synaptic marker, and synaptophysin is a pre-synaptic marker. Co-localized spot with the punctated PSD95 and synaptophysin, indicated by the arrows, might be identified as synapse.

This kit has been optimized with the Thermo Scientific ArrayScan HCS Reader using the Neuronal Profiling BioApplication Software Module, which identifies the synapse measured by colocalization of the pre-synaptic marker with the post-synaptic marker. Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis/management are combined in one high-content analysis (HCA) system to assay for test compounds. In addition to HCS instruments, cells labeled by the kit reagents can be viewed and analyzed by other fluorescence microscopes.

The molecular network between synapses controls synaptic signal transmission and plasticity and regulates neuronal growth, differentiation and death. To understand the relationship between synaptic activity and neuropathophysiology and the molecular mechanism involved in synaptogenesis and synapse regulation, the microstructure of synaptic junctions has been extensively studied. The modulation of neurite and synaptic structures in neurons are closely related to the pathological process of neurological diseases or in neurodevelopment.

Mouse cortical neurons at 16 DIV were stained with the Synaptogenesis kit: Green (PSD95).
Red (Synaptophysin)

   Product Detail 

The Synaptogenesis Kit enables simultaneous detection of neuronal population, neurite, pre-synaptic vesicle, post-synaptic puncta and synapse using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates.