Magic Red Caspases 3 & 7 Detection Kit

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Regular size, ~100 tests:

Trial size, ~25 tests:

Magic Red Caspases 3&7 Detection kit contains:
ICT's red Magic Red caspases 3&7 detection probe, MR-(DEVD)2
Hoechst stain (a blue DNA stain)
Acridine orange (a lysosomal stain)
MR-Caspases 3&7 Apoptosis Manual

Caspases 3&7 are active in the cytosol. After staining with MR, there are often areas of bright red fluorescence located within lysosomal structures. However, caspases are not known to be present within lysosomes.  The presence of the red fluorescent product within the lysosomes is most likely due to the migration and concentration of the cleaved red fluorescent fluorophore from the cytosol into the lysosomes, thereby creating bright spots.

Just add the reagent to media, let it incubate, and read the cells.  If there are active caspase 3&7 enzymes, the reagent will fluoresce red, if not, it will not fluoresce.  Watch 2 movies of real-time caspase activity, courtesy of Dr. M. Purschke, MA General Hospital: movie clip of cells ; movie of fluorescence. ICTs MR-DEVD kits give a clear picture of apoptotic versus non-apoptotic cells and enable you to monitor caspase activity over time.

Magic Red-DEVD kits measure apoptosis via active caspases 3&7 in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. These unique kits are not ELISAs and do not use antibodies - instead they use a cell-permeant substrate sequence, DEVD, specifically targeted by active caspases 3 & 7. This peptide sequence is linked to a red fluorophore, Magic Red, which fluoresces once cleaved by active caspases 3 or 7.  This kit is a good assay to monitor apoptosis over time. 

Assess caspases 3&7 activity in vitro and in real time