Mitotic Index Reagent Kit

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 Mitotic Index Reagent Kit
 Product# Price  Product Name  Pack Size  
         
 K0500011      -    Mitotic Index: Phospho-Histone H3 (green)  5 x 96    SELECT   
 R0105101      -    Mitotic Index: Phospho-Histone H3 (green)  50 x 96    SELECT   
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Figure 2. Variability of response of MDCK cells to different anti-mitotic compounds.

Figure 1. Images taken from the ArrayScan? HCS Reader of MDCK cells, while the right panel was treated with the mitotic inhibitor vinblastine (25 nM for 10 hours). Blue channel indicates nuclei, pink channel indicates mitotic bodies.

In this assay, mitotic cells are selectively identified using an antibody specific for a phosphorylated core histone protein that is abundant in the nuclei of dividing cells.  Many therapeutic agents target cancerous cells by blocking mitotic progression, eventually causing cell death.  Compounds that inhibit mitotic progression, such as nocodazole, vinblastine, colchicine, paclitaxel and curacin, increase the mitotic index of a cell population. The Mitotic Index Kit assay can be used both for primary screening of compounds with anti-mitotic activity and secondary screening for functional analysis of lead compounds. The Mitotic Index Kit, in combination with the ArrayScan HCS Reader and the Mitotic Index BioApplication software enables automated plate handling, focusing, cell image acquisition, analysis and quantification. For a more detailed description of the image processing algorithm, see the Mitotic Index BioApplication Guide that accompanies the Mitotic Index Application software.

Mitotic index, a valuable means of characterizing cell proliferation, represents the cell fraction within a population undergoing cell division. The mitotic index is often higher in cancerous cells because of uncontrolled cell proliferation. Mitotic cells are visually identifiable by the presence of mitosis-specific proteins.  Standard assays determine mitotic index either manually through direct microscopic characterization of tissue samples, or indirectly through analysis of DNA content using fluorescence-activated cell sorting. The Mitotic Index Reagent Kit used in conjunction with the Mitotic Index BioApplication Software (Product No S50-0005-1) and the ArrayScan HCS Reader, enables objective and automatic mitotic index quantitation on a screening scale much faster than manual microscopy methods.

 
Mitotic Index Reagent Kit Features
Cells prepared with the HCS Reagents can be analyzed directly via standard fluorescence microscopy; or HCS imaging systems
Simple sample preparation/processing requires less than 2.5h after compound treatment
Functional whole cell assay is more accurate and biologically relevant than flow cytometric analysis
No cell lysis, purification, or filtration steps
Kit antibody binds to epitope abundant in mitotic cells with high sensitivity and specificity
The Cellomics Mitotic Index Kit enables determination of the percentage of mitotic cells within a population. The assay is performed on live cells growing on standard high-density microplates. The supplied reagents include a primary antibody specific to an epitope present in mitotic cells, a DyLight 488-conjugated Secondary Antibody, Hoechst Dye to identify the nuclear region and various other buffers.

   Product Detail 

The Mitotic Index HCS Reagent Kit provides high-content screening (HCS)-quality fluorescence reagents and a validated protocol for measurement of mitotic index, or the fraction of cells undergoing cell division within a population.