Phospho-p53 (ser15) and p53 Detection Kits

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 Phospho-p53 (ser15) and p53 Detection Kits
 Product# Price  Product Name  Pack Size  
         
 8400501      -    Phospho-p53 (orange) and p53 (green) Activation  1 x 96    SELECT   
 8400502      -    Phospho-p53 (orange) and p53 (green) Activation  5 x 96    SELECT   
 8400701      -    Phospho-p53 (orange) Activation  1 x 96    SELECT   
 8400702      -    Phospho-p53 (orange) Activation  5 x 96    SELECT   
 Details All Product Numbers 

Figure 2. Dose response curves using camptothecin.

Figure 1. Staining of p53 and phospho-p53 in A549 cells treated with media only (untreated) or with 5 ?M camtothecin for 20 hrs (Camptothecin 5?M). Proteins were detected according to the kit protocol. The cells were imaged using a Cellomics ArrayScan HCS Reader.

The Multiplexed and Singleplexed phospho-p53 and p53 Detection Kits have been optimized for use with Cellomics ArrayScan? HCS Reader using the Compartmental Analysis BioApplication Software Module. These kits can be used with other Cellomics BioApplications. Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis/management are combined in one high-content screening (HCS) system to assay for test compounds regulating phospho-p53 and p53 detection. In addition to HCS instruments, cells labeled by the reagents in this kit can be viewed and analyzed by other fluorescence microscopy-based instruments.

The orange phospho-p53 and green p53 multiplex kits contain anti-phospho-p53 (Ser15) and -p53 primary antibodies (rabbit polyclonal and mouse monoclonal, respectively) and secondary antibodies conjugated with orange and green fluorophores (DyLight 549 Goat Anti-Rabbit and DyLight 488 Goat Anti-Mouse antibodies, respectively). The orange phospho-p53 singleplex kits contain a rabbit polyclonal primary antibody towards phospho-p53 and a DyLight 549 Conjugated Goat anti-Rabbit Secondary Antibody.

 
Phospho-p53 (ser15) and p53 Detection Kit Features
Available in multiplex or singleplex configurations
Optimized protocol requiring no cell lysis, purification or filtration steps
Immunofluorescence-based assay provides sensitive results without radioactivity
Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS Imaging Systems including the ArrayScan HCS Reader
Primary antibodies are specific for their targets and have minimal cross-reactivity with other target
These kits allow direct measurements in the nucleus using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibodies are specific for their targets and have minimal cross-reactivity with other targets.

   Product Detail 

The Multiplexed phospho-p53 and p53 Detection Kits are for the simultaneous quantitation of phospho-p53 and p53 expression in the same cell.