NFkB and c-Jun Activation Kits

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 NFkB and c-Jun Activation Kits
 Product# Price  Product Name  Pack Size  
         
 8400301      -    NFkB (orange) & c-Jun (green) Activation  1 x 96    SELECT   
 8400302      -    NFkB (orange) & c-Jun (green) Activation  5 x 96    SELECT   
 8400401      -    NFkB (orange) Activation  1 x 96    SELECT   
 8400402      -    NFkB (orange) Activation  5 x 96    SELECT   
 Details All Product Numbers 
  Details All Product Numbers 
 

Figure 2. Dose Response of NFkB and Phospho-c-Jun activation by TNF-alpha.

Figure 1. Staining of phospho-c-Jun (A, C) and NFB (B, D) in HeLa cells treated with media only (A, B) or with 25 ng/ml TNF-a for 25 min (C, D). Proteins were detected according to the kit protocol. The same cells are shown in panels A and B, and in C and D. The cells were imaged using a Cellomics ArrayScan HCS Reader.

The Multiplexed NFkB and c-Jun Activation Kits have been optimized for use with the Thermo Scientific Cellomics ArrayScan? HCS Reader using the Molecular Translocation BioApplication Software Module. These kits can be used with other Cellomics BioApplications. Thus, automated plate handling, focusing, cell image acquisition/processing, and data analysis/management are combined in one high-content screening (HCS) system to assay for test compounds regulating NFkB and c-jun activation. In addition to HCS instruments, cells labeled by the reagents in this kit can be viewed and analyzed by other fluorescence microscopy-based instruments.

The orange NFkB and green c-jun duplex probes kits contain primary antibodies towards NFkB and phosphorylated c-jun (rabbit polyclonal and mouse monoclonal, respectively) and secondary antibodies conjugated with orange and green fluorophores (DyLight 549 Goat Anti-rabbit and DyLight 488 Goat Anti-mouse Antibodies, respectively). The orange NFkB probe kits contain a rabbit polyclonal primary antibody towards NFkB and a DyLight 549-conjugated Secondary Antibody.

 
NFkB and c-Jun Activation Kit Features
Available in multiplex or singleplex configurations
Optimized protocol requiring no cell lysis, purification or filtration steps
Immunofluorescence-based assay provides sensitive results without radioactivity
Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS Imaging System including the ArrayScan HCS Reader
Primary antibodies are specific for their targets and have minimal cross-reactivity with other targets   
These kits allow direct measurements of NFkB and phospho-c-jun translocation from the cytoplasm to the nucleus using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibodies are specific for their targets and have minimal cross-reactivity with other targets.

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The Multiplexed NFkB and c-Jun Activation HCS Reagent Kits provide a highly effective tool for the simultaneous quantification of NFkB and cjun activation in the same cell.