Caspase 10 Detection Kit

It will not work in previously fixed cells or paraffin cells, as that treatment inactivates the caspase 10 enzyme.  See our accessories page for a caspase 10 antibody and other reagents.

FLICA kits have been used successfully with many types of cancer cells; epithelial cells; endothelial cells; macrophages; and other types of human, mouse, rat tissues, and many other species.

The caspase 10 FLICA reagent is cell permeant, so you dont have to lyse or permeabilize the membrane to get it into the cell. Just culture your cells, add the FLICA probe to the media, incubate, and wash the cells. If there are active caspase 10 enzymes, they will bind to FLICA and retain it within the cell, thereby capturing the fluorescent signal. The FLICA probe constantly fluoresces, therefore any unbound reagent must be washed back out of the cell to remove any background noise.  This is done by several quick rinse and spin steps, or further incubation with the wash buffer or cell culture media. Click here to read our complete manual.

ICT's caspase 10 inhibitor probe is comprised of 3 segments:
a green fluorescent label, FAM (carboxyfluorescein)
an inhibitor peptide sequence targeted by active caspase 10 (AEVD)
and a fluoromethylketone group (FMK), which forms a covalent bond with the active enzyme.
FLICA kits have been used successfully with a multitude of cell lines from a variety of species (human, mouse, rat, etc), including, but not limited to, cancer cells, neurons, primary cells, epithelial cells, endothelial cells, and macrophages. For in vitro apoptosis detection via active poly caspases, see our FLICA Poly Caspases Detection Kits.

The FLICA assay also works with adherent cells and very thin tissue sections. FLICA will not work in previously fixed cells or paraffin cells, as those treatments inactivate the caspase 10 enzyme. Please call for details or technical questions at 800-829-3194.

ICT's FLICA caspase 10 detection assay is a simple yet accurate method to measure caspase 10 activity in whole cells. Simply add the FLICA caspase 10 reagent to the media and incubate. Cells containing active caspase 10 will fluoresce green. The kit contains:
ICT's FLICA caspase 10 detection probe, FAM-AEVD-FMK
10x apoptosis wash buffer
Hoechst stain (a blue DNA stain)
Propidium iodide (a red live/dead stain)
Fixative (for use after labeling with FLICA)
Trial size, ~25 tests:
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Regular size, ~100 tests:
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Caspase 10 activity may be detected in individual cells or suspensions of up to 1x10^6 cells/mL. The noncytotoxic FLICA probe is cell permeant: no lysis or permeabilization steps are necessary. Just culture cells, add FLICA to the media, incubate, and wash. Active caspase 10 enzymes will bind to FLICA and retain it within the cells, thereby capturing the green fluorescent signal. FLICA constantly fluoresces, therefore any unbound reagent must be washed out of the cell to remove any background noise. The resulting positive fluorescent signal can be detected with a fluorescence microscope, a fluorescence plate reader, or a flow cytometer. The green FLICA probe, FAM, excites at 490nm and emits at 520nm.