RNA Primers

5' or 3' CY5 NHS Ester

3' or Int CY3

5' CY3

5' or 3' JOE NHS Ester

5' or 3' ROX NHS Ester

5' or 3' TAMRA NHS Ester

5' HEX

5' TET

5'or 3' 6-FAM

Fluorophores

 

 

5' TAMRA NHS Ester-3' Dabcyl

5' or 3' -S-S or -SH

5' HEX-3' Dabcyl

5' or 3' DIG

5' 6-FAM-3' Dabcyl

5' or Int Biotin dT

Molecular Beacons

5' or 3' Biotin

 

5' NH2 C12

3' Eclipse

5' or 3' NH2 C6

3' BHQ-2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Attachment  Chemistry & Linkers   Linkers Linkers

3' BHQ-1

 

5' Dabcyl NHS Ester

5' or Int dSpacer

3' Dabcyl

5' or Int Spacer 18

Dark Quenchers

5' or Int Spacer 9

 

C3 Spacer

5' CY5 NHS Ester-3' BHQ-2

Spacers

5' CY3 NHS Ester-3' BHQ-2

 

5' ROX NHS Ester-3' BHQ-2

3' Dideoxy-C(ddC)

5' TAMRA NHS Ester-3' BHQ-2

Locked Nucleic Acids (LNA)

5' HEX-3' Eclipse

5-Methyl dC

5' HEX-3' BHQ-1

3' Inverted dT

5' HEX-3' TAMRA NHS Ester

Modified bases

5' TET-3' TAMRA NHS Ester

 

5' 6-FAM-3' Eclipse

5' or 3' Phosphorylation

5' 6-FAM-3' BHQ-1

Phosphorylation

5' 6-FAM-3' TAMRA NHS Ester

 

Dual-Labeled DNA Probes

 

 

 

 

 

 

 

 

 

 

 

 

 

dU or dI

Modification & Label

5

2

 (90?120 base)

Synthesis of long chain

5

2

(60?90 base)

Synthesis of long chain

100

4

100

4

50

3

30

3

20

3

5?10

dual PAGE & HPLC

 

HPLC

 

PAGE

 

OPC

 

3

5

(60 base)

Synthesis of general primers

 

methods

Purification

(Working days)

Delivery times

(O D s)

Yield

the content of Synthesis

Regular Oligos

 

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