The quality of 96-Well Gel/PCR DNA Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragments of various sizes. The purified DNA is checked by agarose gel analysis. The entire procedure can be completed in 30 minutes. 2. Following washing of contaminants, the purified DNA is eluted by low salt buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reactions mixtures without toxic phenol extraction and alcohol precipitation. 1. The method utilizes a chaotrophic salt to denature enzymes and in this condition DNA fragment is absorbed to the fibre matrix in each well of the plate.
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Operation: Centrifuge / vacuum manifold Operation time: 30 minutes |