ATP Gel/PCR DNA Fragments Extraction Kit

The quality of ATP Gel/PCR fragments Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragment of various sizes from either aqueous solution or agarose. The purified DNA is checked by agarose gel analysis.

The entire procedure does not require DNA phenol extraction and alcohol precipitation , and could be completed in 20 minutes .

4.  After washing off contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water.

3.  Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture.

2.  The DNA fragments in the chaotropic salt are then bound to the uniquely designed glass fiber matrix in the spin column in the optimized salt concentration and pH provided by our buffer.

1.  The method uses a chaotropic salt,guanidine thiocyanante , to dissolve the agarose gel and denature enzymes.

Introduction

Format: Spin column Sample: Up to 300 mg agarose gel slice; Up to 100 ?l PCR product or other enzymatic reaction Operation: Centrifuge / vacuum manifold DNA size: 50 bp ~ 10 kb Operation time: 20 minutes for gel extraction; 15 minutes for PCR clean up Expectant recovery: 75-85% for gel extraction; 85-95% for PCR clean up Application: DNA Sequencing; Ligation; PCRRestriction Digestion; DNA labeling   ATP Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments ( 50 bp-10 kb ) from agarose gel, PCR or other enzymatic reaction.