7AAD fluorescent vital stain

ICT is offering a new line of active cathepsin enzymes and related proteases purified from human liver or neutrophils. Use them for kinetic studies, to cleave target substrates, or to screen for inhibitors.

Camptothecin isolated from the Chinese herb xi shu (Camptotheca acuminata); an inhibitor of DNA topoisomerase I and inducer of apoptosis.
Granzyme B human recombinant expressed from human cDNA (residues Ile-21-Tyr-247); 25.4 kDa.
Staurosporine isolated from Streptomyces sp.; a protein kinase C inhibitor. Cytoplasmic proteins directly modulated by staurosporine may be involoved in signaling the induction of DNA fragmentation and apoptosis.
TNF alpha human recombinant expressed in E. coli as a single, non-glycosylated, polypeptide of 158 amino acids; 17.5 kDa; >/=98% purity by SDS-PAGE, RP-HPLC, and FPLC. Fully biologically active.
TNF beta human recombinant expressed in E. coli as a single, non-glycosylated, polypeptide of 171 amino acids; 18.6 kDa; >/=98% purity by SDS-PAGE, RP-HPLC, and FPLC. Fully biologically active.
TRAIL (Apo2L) human recombinant expressed in E. coli comprising residues 114-281 of the soluble domain; 22 kDa; >95% purity by SDS-PAGE. Fully biologically active. Induced apoptosis at 1ug/mL in U937 and Jurkat cells within 5 hours.
Do kinetic studies with purified proteases
ICT is offering a new line of highly active caspase enzymes for high-throughput screening, cleavage studies, and enzyme kinetics, among other applications. Most are >90% pure by SDS-PAGE. 1 U = 1 pmol/min at 30C using the substrates listed.

7AAD is a red fluorescent vital stain. Dead cells, and membrane-compromised cells stain red.
Acridine Orange (266 ug/mL) is a red/orange fluorescent stain. It is a chelating dye and can be used to reveal lysosomes, nuclei, and nucleoli.
CFSE is a green fluorescent membrane stain that can be used in cell proliferation assays. It binds to membrane proteins.
DAPI is a blue fluorescent stain that forms complexes with natural double-stranded DNA. Use it to detect DNA.
Hoechst 33342 ( 200 ug/mL) is a blue fluorescent stain that binds to DNA .
Propidium Iodide (250 ug/mL) is a red vital stain. It stains necrotic, dead, and membrane-compromised cells red.
10X PBS is a concentrated buffer that can be used to wash cells, run cells through in flow cytometry, dialyze proteins, and run Protein G columns.
Make positive controls and study enzyme kinetics by inducing apoptosis
ICT is offering a new line of reagents to induce apoptosis, study enzyme kinetics, cleave substrates, and screen for inhibitors. (Please contact us for pricing.)

Distinguish apoptotic cells from necrotic cells thanks to our new line of accessory stains, dyes, and reagents. When used in combination with ICTs fluorescent detection kits, they can be used to distinguish apoptotic cells from membrane-compromised and necrotic cells. Using this items together in dual staining experiments will save time and enhance your data. (Please contact us for pricing.)