T4 DNA Ligase Molecular Reagents

T4 DNA ligase catalyzes the formation of a phosphodiester bonds between 5 phosphate and 3 hyroxyl termini in duplex DNA/RNA. This enzyme can join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrid.

Source : T4 DNA ligase is isolated from a recombinant E.coli strains.

Unit definition : One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of Lambda DNA 30 minute at 16
  • Country:India
  • telephone:91-011-25594124
T4 DNA ligase catalyzes the formation of a phosphodiester bonds between 5 phosphate and 3 hyroxyl termini in duplex DNA/RNA.
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