T4 DNA ligase catalyzes the formation of a phosphodiester bonds between 5 phosphate and 3 hyroxyl termini in duplex DNA/RNA. This enzyme can join blunt end and cohesive end termini as well
as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrid. Source : T4 DNA ligase is isolated from a recombinant E.coli strains. Unit definition : One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of Lambda DNA 30 minute at 16 |
T4 DNA ligase catalyzes the formation of a phosphodiester bonds between 5 phosphate and 3 hyroxyl termini in duplex DNA/RNA. |