E. coli uracil-DNA Glycosylase(UDG) catalyzes the release of Free Uracil from Uracil containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double stranded DNA, but not
from oligomers (6 or less bases). SPECIFICATIONS : Enzyme is supplied at a Concentration of 20U/?l along with one vial of 1X reaction buffer. STORAGE BUFFER : 20mM Tris-HCL (pH 8.0), 50 mM NaCl, 0.1mM EDTA, 50% glycerol. SUPPLIED REACTION BUFFER (10X) : 20mM Tris HCl(pH 8.0) 1mM EDTA. STORAGE : Recommended storage is at 20 |
E. coli uracil-DNA Glycosylase(UDG) catalyzes the release of Free Uracil from Uracil containing DNA. |