M-MuLV Reverse Transcriptase, Rnase H Minus Molecular Reagents
Product Details
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M-MuLV Reverse transcriptase is purified from E. Coli strain harboring a plasmid that directs the synthesis of modified form of Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase.
M-MuLV is a RNA/ DNA Directed DNA Polymerase. The enzyme can synthesize complementary DNA strand initiating from a primer using either RNA (cDNA Synthesis) or single stranded DNA as
template. This enzyme had been genetically altered to remove associated RNase H activity. Removal of RNase H activity resulted in an increase of full-length cDNA products. MW of Reverse is
69 kDa. SPECIFICATIONS : Enzyme is supplied at a Concentration of 200U/?l along with one vial of 5 X buffer, one vial of additional 25mM MgCl2 & one vial of DNase free water. STORAGE BUFFER : 50mM TrisHCL (pH 8.3), 1mM EDTA, 100mM NaCl, 0.1mM DTT, 0.1% Triton X-100, 50% glycerol. SUPPLIED REACTION BUFFER : (5 X) 250mM Tris HCL(pH 8.3), 500mM KCL, 50mM DTT, 15mM MgCl2 STORAGE : Recommended storage is at -20 |
- Country:India
- telephone:91-011-25594124
| M-MuLV Reverse transcriptase is purified from E. Coli strain harboring a plasmid |
