Cell Spreading Reagent Kit

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Our Cell Spreading Reagent Kit (K0600011 and R0200031) has been replaced by our Whole Cell Stain Toolbox Kit. Our Whole Cell Stain Toolbox kit contains Whole Cell Stain Green (8403201) that provides uniform staining of the cell volume, and the Hoechst nuclear stain to enable identification of cells nuclei.  In addition, it contains the various buffers needed for effective cell staining, and a complete labeling protocol.  The old Cell Spreading Reagent Kit (K0600011 and R0200031) used fluorescent phalloidin to label F-actin and to define the cell area; the replacement Whole Cell Stain Toolbox Kit offers more uniform staining of the cellular cytoplasm enabling accurate segmentation by image processing software of touching cells.  In addition, the cell labeling time is shorter (30-45 minutes) that with the old kit (1.5 hours).  For customers wanting to label F-actin with phalloidin, the new Cytoskeletal Rearrangement: F-actin kits (8402501 and 8402502) provide more versatile options. 
 
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Cell attachment and spreading are multifactorial events mediated by interactions between adhesion molecules on the cell surface and underlying extra cellular matrices. Regulation of these events is central to a number of physiological processes including cancer cell metastasis, inflammation, and angiogenesis. Rather than measuring cell-ligand binding constants, this assay measures the ability of cells to respond by attaching and spreading on a specific substrate, providing a functional assay of the regulatory and structural molecules needed for morphological changes in the cell. The efficacy of drugs that target the function or expression of these molecules can be measured by the rate of attachment and spreading on a tissue-specific substrate.