Cell Motility Reagent Kit

ORDER NOW - Cell Motility Reagent Kit
 
 
 Cell Motility Reagent Kit
 Product# Price  Product Name  Pack Size  
         
 K0800011      -    Cell Motility  5 x 96    SELECT   
 R0200051      -    Cell Motility  50 x 96    SELECT   
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Figure 2. Dose response curve for cell motility stimulation in L9292 cells.

Figure 1. Images of tracks (black) generated by A549 cells in the absence (unstimulated, left) or presence (stimulated, right) of serum. Cells appear as red fluorescent spots due to the cytoplasmic staining (rhodamine-conjugated phalloidin). Images were acquired by the ArrayScan HCS Reader.

The Cell Motility Kit facilitates the quantification of cell movement with images of fixed cells. This functional assay can predict the efficacy of potential drugs for a number of therapeutic areas. The assay is performed by plating cells on a lawn of microscopic fluorescent beads. As cells move across the lawn, they phagocytose and push aside the beads, clearing phagokinetic tracks behind them. The track area is proportional to the magnitude of cell movement. The reagents in this kit combined with the ArrayScan HCS Reader and the Cell Motility BioApplication Software enable automated plate handling, focusing, cell image acquisition, analysis and quantification. For a more detailed description of the image-processing algorithm, see the Cell Motility BioApplication Guide that accompanies the Cell Motility BioApplication software.

Cell motility is central to a number of biological and pathologic processes including cancer cell invasion and metastasis, inflammation, angiogenesis, wound repair, and embryonic development. The movement of cells occurs via the concerted activities of cell adhesion molecules, the actin cytoskeleton and an extensive network of signaling molecules. A combination of cell substrate adhesion, cell spreading, lamellar protrusion and directional control work together for net translocation. These activities are regulated both by external factors including extracellular matrix proteins, cytokines and soluble growth factors, and by intracellular signal transduction cascades.

 
Cell Motility Reagent Kit Features
Versatile assay is widely applicable to a variety of cell types
Validated protocol for sample preparation/processing requires 2.5 hours post-compound incubation
Assay utilizes fluorescence reagents and requires no cell lysis, purification or filtration steps; and no handling of radioactive isotopes
Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or any HCS imaging systems
The Cell Motility HCS Reagent Kit provides reagents and a protocol optimized for quantification of cell motility by directly measuring the size of tracks generated by migrating cells. The assay is performed on live cells growing on standard high density microplates. The kit includes Blue Fluorescent Beads, Rhodamine-conjugated Phalloidin for staining the cytoskeleton and assay buffers.

   Product Detail 

The Cell Motility HCS Reagent Kit provides high-content screening (HCS)-quality fluorescence reagents and a protocol optimized for visualizing and quantifying cell movement by directly measuring the size of tracks generated by migrating cells.