MDM2 and p53 Detection Kits

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 MDM2 and p53 Detection Kits
 Product# Price  Product Name  Pack Size  
         
 8401801      -    MDM2 (green) and p53 (orange) Detection  1 x 96   SELECT   
 8401802      -    MDM2 (green) and p53 (orange) Detection  5 x 96   SELECT   
 8401901      -    MDM2 (orange) Detection  1 x 96   SELECT   
 8401902      -    MDM2 (orange) Detection  5 x 96   SELECT   
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See product instructions for additional background, example data and literature references.

Figures 3. Detection and analysis of MDM2 and p53 in A549 cells in response to treatment with Nutlin-3. Images are of cells treated with 6 ?Mnutlin-3 for 20 hours. Nuclear morphology detected with Hoechst dye (left). Detection of MDM2 (green) and p53 (orange)(right).

Figure 2. Staining of MDM2 and p53 in A549 cells treated with 1.5 ?M camptothecin for 20 hours. Except for the Hoechst nuclei control (left) non-treated cells had negligible staining of either MDM2 or p53. Proteins were detected according to the kit protocol. The cells were imaged using a Cellomics ArrayScan HCS Reader.

These kits have been optimized for use with the ArrayScan? HCS Reader using the Compartmental Analysis BioApplication Software Module but can be used with other Cellomics BioApplications. Thus, automated plate-handling, focusing, cell image acquisition/processing and data analysis/management are combined in one high-content screening (HCS) system to assay for test compounds regulating MDM2 and p53 expression. In addition to HCS instruments, cells labeled by the reagents in this kit can be viewed and analyzed by other fluorescence microscopes.

The orange p53 and green MDM2 multiplex kits contain primary antibodies toward p53 and MDM2 rabbit polyclonal and mouse monoclonal, respectively and secondary antibodies conjugated with DyLight 488 (green) and DyLight 549 (orange) Fluorophores. The orange MDM2 singleplex kits contain a mouse monoclonal primary antibody toward MDM2 and a DyLight 549-Conjugated Secondary Antibody.

   Product Detail 

The MDM2 and p53 Detection HCS Reagent Kits are for the simultaneous quantitation of MDM2 and p53 expression in the same cell. These kits allow direct measurements of MDM2 and p53 in the nucleus using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibodies are specific for their targets and have minimal cross-reactivity with other targets.