Cell Viability Reagent Kit

Figure 1. Live and dead cells distinguished by the ArrayScan HCS Reader and the Cell Viability Kit Reagents. HeLa cells were plated in a 96-well microplate and then treated with the Positve Control Compound (supplied with the kit). Hoechst staining identifies all cells in the imaged field (A). VitalDye (green) and DeadDye (red) show live and dead cells, respectively (B).

The Cell Viability Kit Reagents, in combination with the ArrayScan HCS System and the Cell Viability Application software enable automated plate handling, focusing, cell image acquisition, and analysis and cell viability quantification. For a more detailed description of the image processing algorithm, see the Cell Viability Application Guide that accompanies the software.

When cells are dead or dying, plasma membranes leak and become permeable to large macromolecules. In this state, DeadDye enters the cell and is visualized by its red fluorescence emission. VitalDye, a membrane-permeable fluorescent probe, operates as a live-cell indicator. Soluble cytoplasmic enzymes cleave VitalDye, trapping its fluorescent moiety inside intact cells. Live cells stained with VitalDye are distinguished from dead cells by their green fluorescence emission. Identification of the entire cell population is achieved using Hoechst Dye, a membrane-permeable nucleic acid stain emitting in the blue fluorescence channel. DeadDye and VitalDye may be used separately or in combination to quantify dead cells, live cells or both cell populations.

 
Cell Viability Reagent Kit Features
Assay is versitle and widely applicable to a variety of cell types
Utilizes fluorescent reagents and requires no cell lysis, purification, or filtration steps; and no handling of radioactive isotopes
Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS imaging systems.
DeadDye and VitalDye fluorescent probes offer sensitivite, quantitative and qualitative screening results without radioactivity  
The Cellomics Cell Viability Reagent Kit for high-content screening enables quantification of live and dead cells, and total cell counts. The kit provides a fixed end-point assay based on fluorescence detection of live and dead cells distributed on microplates. The core reagents supplied include DeadDye and VitalDye. The nuclei are identified by DNA-specific Hoechst Dye, which is also included in the kit.

   Product Detail 

The Cellomics Cell Viability HCS Reagent Kit provides high-content screening(HCS)-qualified fluorescence reagents and a validated, optimized protocol for cytotoxicity screening, using either a Cellomics fully automated HCS Reader, other HCS imaging system, or standard fluorescence microscope. At the core of the Cell Viability assay are DeadDye and VitalDye proprietary fluorescent probes that enable quantification of the number of live and dead cells, as well as their relative percentages and live/dead ratio, on any standard microplate format.