BrdU and Ki67 Cell Proliferation Kits

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 BrdU and Ki67 Cell Proliferation Kits
 Product# Price  Product Name  Pack Size  
         
 8401101      -    BrdU (green) and Ki67 (orange) Cell Proliferation  1 x 96    SELECT   
 8401102      -    BrdU (green) and Ki67 (orange) Cell Proliferation  5 x 96    SELECT   
 8401201      -    BrdU (orange) Cell Proliferation  1 x 96    SELECT   
 8401202      -    BrdU (orange) Cell Proliferation  5 x 96    SELECT   
 8401301      -    Ki67 (orange) Cell Proliferation  1 x 96    SELECT   
 8401302      -    Ki67 (orange) Cell Proliferation  5 x 96    SELECT   
 Details All Product Numbers 

Figure 1. Staining of BrdU and Ki67 in A549 cells (upper panels, 20X objective) and A431 cells (lower pannels, 10X objective). Cells were stained with DAPI (nuclei). Markers were detected according to the kit protocol. The cell images were acquired using a Cellomics ArrayScan HCS Reader. BrdU positive cells are in S phase, Ki67 positive cells are in either G1, S, G2 or M phases and only DAPI positive cells are in G0 phase.

These kits have been optimized with the Cellomics ArrayScan? HCS Reader using the Target Activation BioApplication Software Module but can be used with other Cellomics BioApplications. Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis/management are combined in one high content screening (HCS) system to assay for test compounds regulating BrdU incorporation and the level of Ki67 nuclear antigen. This assay is sensitive, rapid and easy to use. The evaluation of cell proliferation and other information such as cell number, morphology and analysis of other cellular antigens can be obtained from a single culture. In addition to HCS instruments, cells labeled by the kit reagents can be viewed and analyzed by other fluorescence microscopes.

Typical assays that use antibodies to detect DNA-incorporated BrdU require DNA denaturation using harsh treatments such as acid, base or heat. These treatments could result in destruction of cellular morphology, cell integrity and the epitopes of cell surface proteins and other markers. In contrast, HCS Reagent Kits detect BrdU in nuclear DNA without harsh treatments, enabling multiplexed detection of BrdU with antibodies towards other cellular targets.

The multiplex kits contain primary antibodies toward BrdU and Ki67 (mouse monoclonal and rabbit polyclonal, respectively) and secondary antibodies conjugated with DyLight 488 (green) and DyLight 549 (orange) Fluorophores. The orange BrdU singleplex kit contains a mouse monoclonal primary antibody toward BrdU and a DyLight 549-Conjugated Secondary Antibody. The orange Ki67 probe kit contains a rabbit polyclonal primary antibody toward Ki67 and a DyLight 549-Conjugated Secondary Antibody.

BrdU and Ki67 Cell Proliferation Kit Benefits
Analyze single pathway of choice or multiple related pathways in a single assay
Kits have been validated in HCS assays for reliable and reproducible results
Kits work on all HCS platforms offering flexibility in end-user instrumentation
Can purchase only the reagents they need for your needs and reagents for large screening applications
Offer ease-of-use, no optimization or assay development required which saves valuable research time
These kits allow direct measurements of BrdU incorporation and Ki67 expression using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibodies are specific for their targets and have minimal cross-reactivity with other targets.

 
BrdU and Ki67 Cell Proliferation Kit Features
Kits are available in multiplex or singleplex configurations
Robust assays Z values are >0.3 for multiplexed and singleplexed assays
Validated on Cellomics ArrayScan HCS readers: also works on other HCS platforms and fluorescent microscopes
Customized components and bulk quantities available
Optimized protocol and reagent preparations included

   Product Detail 

The BrdU and Ki67 Cell Proliferation HCS Reagent Kits are for simultaneous quantification of DNA replication and Ki67 proliferation marker in the same cell.