Multiplex Mitosis-Apoptosis Kit

Figure 2. Drugs induce Caspase 3 and p53 but inhibit cell proliferation in A549 cells. Different drugs were added to the cells at the indicated concentrations. Each data point represents eight wells from three plates. BrdU, caspase 3 and p53 were measured using the output parameter of nuclear fluorescence intensity with Cellomics Compartmental Analysis Bioapplication. Values are normalized with the maximum control value and presented as percent control.
 
 
 Multiplex Mitosis-Apoptosis Kit
 Product# Price  Product Name  Pack Size  
         
 8408202      -    Multiplex Mitosis-Apoptosis Kit   5 x 96   SELECT   
 8408203      -    Mitosis-Apoptosis Multiplex Kit  50 x 96   SELECT   
 Details All Product Numbers 

Figure 1. Staining of treated and non-treated A549 cells (10X objective). Markers were detected according to the kit protocol. The cells were treated with 1.6 ?M actinomycin D, pulse-labeled with BrdU for 30 minutes and probed with BrdU, active caspase 3 and p53 antibodies followed by corresponding DyLight Secondary Antibodies. Cell images were acquired using the ArrayScan HCS Reader.

Cell proliferation and apoptosis, which denote life and death of the cell, are critical areas of cell biology and drug-discovery research. This kit identifies cell proliferation (BrdU), caspase-dependent cell death pathway and p53 pathway in the cell, which enables systematic investigation of apoptosis and mitosis events.

This kit has been optimized with the Thermo Scientific ArrayScan HCS Reader using the Compartmental Analysis BioApplication Software Module. Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis/management are combined in one high-content screening (HCS) system to assay for test compounds. In addition to HCS instruments, cells labeled by the kit reagents can be viewed and analyzed by other fluorescence microscopes.

 
This kit allows simultaneous measurements of cell proliferation (cell number, DNA replication) and apoptosis using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. DAPI is a DNA-binding dye used to determine the nuclear size and nuclear morphology as well as cell cycle phases by DNA content. The primary antibodies are specific for their targets and have minimal cross-reactivity.

   Product Detail 

The Multiplex Mitosis-Apoptosis Kit is for simultaneous quantification of nuclear DNA content, BrdU incorporation and active caspase 3 and p53 proteins.