1. Select 60-mers from any organism of choice
2. Oligos should also include the appropriate controls (i.e. different organism).
3. All oligos should map to the 3' end of cDNAs
4. All oligos should map to within 1,000 nt of the 3' cDNA end
5. All oligos should correspond to the coding strand (protein strand)
6. Avoid sequence repeats and stretches of polyA, G, C and T
7. Avoid extremes of Tms (mixed sequence composition best)
8. Run against the database to avoid cross-reactivity (<70% sequence identity). Blast Button"
TeleChem International, Inc. Oligo Design Directive Steps 1-8.
Please write to us if you would like to evaluate the program using your own sequences:
For a FREE demo, .
1. Buy the Array Designer Software for only $4,885.00 (Catalog# ADS3)
OR
2. Hire TeleChem/arrayit.com for $20.00 per oligo.
There are two ways to get your oligos designed for your arrays:
Dont pay large upfront costs to bioinformatics companies that will not allow you to own your sequences and discoveries!
For this reason, Arrayit Corporation and PREMIER Biosoft International have joined forces to make state-of-the-art oligonucleotide design software available to the researcher at an affordable price.
Applications include:
Clone mapping
Deletion studies
Gene expression
Gene mapping
Gene re-sequencing
SNP analysis
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