AG-
DNA Recovery | Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
Equipment | Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
Sample Sources | Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
DNA Purity | High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
Storage | Eluted DNA should be stored at ≤ -20ºC. |
Recovery Volume | ≥ 35 μl ofDNA Elution Bufferor DNase free water. |
Processing Volume |
Plasma – Single centrifugation: up to 3 ml Plasma – Double centrifugation: up to 10 ml Serum/Amniotic fluid/Cerebrospinal fluid (CSF) – up to 10 ml Saliva – up to 1 ml Cell-free Saliva – up to 5 ml |