Hipure RNAse A

HiPure RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.

Specific Activity:>2500 u/mg protein (>50 Kunitz units/mg protein)

Form:Contains 1gram HiPure RNase A, Lyophilized Powder, and 100ml Storage Buffer

Features

The HiPure RNase A is free of DNase activity. It is not necessary to heat it before use.

Applications

•Plasmid and genomic DNA preparation
•Removal of RNA from recombinant protein preparations.
•Ribonuclease protection assays
•Mapping single-base mutations in DNA or RNA

Storage

Store the HiPure RNase A powder at 4ºC or -20℃. Dissolved RNase A solution should be stored at -20ºC.