Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA KitBasic Information

The ELISA kit is a Sandwich enzyme immunoassay technique for the quantitative determination of δ-Aminolevulinic Acid Dehydratase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

δ-Aminolevulinic Acid DehydrataseIntroduction

ALAD synthesizes porphobilinogen through the asymmetric condensation of two molecules of aminolevulinic acid. All natural tetrapyrroles, including hemes, chlorophylls and vitamin B12, share porphobilinogen as a common precursor. Porphobilinogen synthase is the prototype morpheein. PBGS is encoded by a single gene and each PBGS multimer is composed of multiple copies of the same protein. Each PBGS subunit consists of a ~300 residue αβ-barrel domain, which houses the enzyme's active site in its center, and a >25 residue N-terminal arm domain. Allosteric regulation of PBGS can be described in terms of the orientation of the αβ-barrel domain with respect to the N-terminal arm domain.

Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit Test Method

This ELISA kit uses the Sandwich-ELISA principle. The micro plate provided in this kit has been pre-coated with an antibody specific to Sheep δ-Aminolevulinic Acid Dehydratase. Standards or samples and Horseradish Peroxidase (HRP) labeled detection antibody specific for Sheep δ-Aminolevulinic Acid Dehydratase are added to the plate wells together and incubated. After washing off unbound material, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Sheep δ-Aminolevulinic Acid Dehydratase in the samples is then calculated from the OD value by establishing a standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000148218 - Ensembl, May 2017

2. GRCm38: Ensembl release 89: ENSMUSG00000028393 - Ensembl, May 2017

3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.