Rat 4-HNE(4-Hydroxynonenal) ELISA Kit Basic Information

Feiyue's Rat 4-HNE(4-Hydroxynonenal) ELISA Kit is an ELISA reagent for detection of 4-HNE(4-Hydroxynonenal), plasma or cell with sensitivity, specificity and consistency.

4-HNE(4-Hydroxynonenal) Introduction

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an α,β-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.4-HNE has 3 reactive groups: an aldehyde, a double-bond at carbon 2, and a hydroxy group at carbon 4.4-Hydroxynonenal is found throughout animal tissues, and in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction, due to the increase in stress events.

Rat 4-HNE(4-Hydroxynonenal) ELISA Kit Test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. T he concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve

Reference:

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4. The August 2003 number of the journal Molecular Aspects of Medicine was entirely dedicated to 4-hydroxy-trans-2-nonenal.

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