Product Name | Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit |
Catalog NO. | FY-ESH4795 |
Alias | ALAD |
Application |
The ELISA kit is a Sandwich enzyme immunoassay technique for the quantitative determination of Sheep δ-Aminolevulinic Acid Dehydratase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Size | 48T, 96T |
Storage | 2-8 ℃ for 6 months |
Sensitivity | 2 pg/mL |
Species | Sheep |
Detection Range |
2-64 pg/mL |
CV(%) |
Intra-Assay: CV<10% Inter-Assay: CV<12% |
Note | For Research Use Only |
The ELISA kit is a Sandwich enzyme immunoassay technique for the quantitative determination of δ-Aminolevulinic Acid Dehydratase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
ALAD synthesizes porphobilinogen through the asymmetric condensation of two molecules of aminolevulinic acid. All natural tetrapyrroles, including hemes, chlorophylls and vitamin B12, share porphobilinogen as a common precursor. Porphobilinogen synthase is the prototype morpheein. PBGS is encoded by a single gene and each PBGS multimer is composed of multiple copies of the same protein. Each PBGS subunit consists of a ~300 residue αβ-barrel domain, which houses the enzyme's active site in its center, and a >25 residue N-terminal arm domain. Allosteric regulation of PBGS can be described in terms of the orientation of the αβ-barrel domain with respect to the N-terminal arm domain.
This ELISA kit uses the Sandwich-ELISA principle. The micro plate provided in this kit has been pre-coated with an antibody specific to Sheep δ-Aminolevulinic Acid Dehydratase. Standards or samples and Horseradish Peroxidase (HRP) labeled detection antibody specific for Sheep δ-Aminolevulinic Acid Dehydratase are added to the plate wells together and incubated. After washing off unbound material, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Sheep δ-Aminolevulinic Acid Dehydratase in the samples is then calculated from the OD value by establishing a standard curve.
Reference:
1. GRCh38: Ensembl release 89: ENSG00000148218 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028393 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
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