Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit

FOB Price
USD $50.00 / Piece
Place Of Origin
中国
Minimum Order
1
Packaging
96T
Delivery
5 Days

Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA KitBasic Information

Product Name Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit
Catalog NO. FY-ESH4795
Alias ALAD
Application
The ELISA kit is a Sandwich enzyme immunoassay technique for the quantitative determination of Sheep δ-Aminolevulinic Acid Dehydratase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Size 48T, 96T
Storage 2-8 ℃ for 6 months
Sensitivity 2 pg/mL
Species Sheep
Detection Range
2-64 pg/mL
CV(% Intra-Assay: CV<10%
Inter-Assay: CV<12%
Note For Research Use Only

The ELISA kit is a Sandwich enzyme immunoassay technique for the quantitative determination of δ-Aminolevulinic Acid Dehydratase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

δ-Aminolevulinic Acid DehydrataseIntroduction

ALAD synthesizes porphobilinogen through the asymmetric condensation of two molecules of aminolevulinic acid. All natural tetrapyrroles, including hemes, chlorophylls and vitamin B12, share porphobilinogen as a common precursor. Porphobilinogen synthase is the prototype morpheein. PBGS is encoded by a single gene and each PBGS multimer is composed of multiple copies of the same protein. Each PBGS subunit consists of a ~300 residue αβ-barrel domain, which houses the enzyme's active site in its center, and a >25 residue N-terminal arm domain. Allosteric regulation of PBGS can be described in terms of the orientation of the αβ-barrel domain with respect to the N-terminal arm domain.

Sheep δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit Test Method

This ELISA kit uses the Sandwich-ELISA principle. The micro plate provided in this kit has been pre-coated with an antibody specific to Sheep δ-Aminolevulinic Acid Dehydratase. Standards or samples and Horseradish Peroxidase (HRP) labeled detection antibody specific for Sheep δ-Aminolevulinic Acid Dehydratase are added to the plate wells together and incubated. After washing off unbound material, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Sheep δ-Aminolevulinic Acid Dehydratase in the samples is then calculated from the OD value by establishing a standard curve.


Reference:

1. GRCh38: Ensembl release 89: ENSG00000148218 - Ensembl, May 2017

2. GRCm38: Ensembl release 89: ENSMUSG00000028393 - Ensembl, May 2017

3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

  • Country:
  • Business Type:
  • Market:
  • Founded Year:
  • Address:
  • Contact:FeiyueBio Stella
*Your name:
*Your Email:
*To:Wuhan Feiyue Biotechnology Co., LTD.,
*Subject:
*Message:
Enter between 20 to 3,000 characters. English only.     Characters : 0 / 3000
*Enter the secure code shown below Mfrbee security Image      Reload Image

submiting now We do inquire for you , please wait ...

Bovine δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit
Bovine δ-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit

Wuhan Feiyue Biotechnology Co., LTDlocated in Wuhan East Lake New Technology Development Zone with registered capital of 15 million yuan. Focusing on the research and development, production, sales and import and export of biological research reagents and primary and secondary ...

Wuhan Feiyue Biotechnology Co., LTD