Human Tau Protein (MAPT) ELISA kit

Human Tau Protein (MAPT) ELISA kit

Catalog No:FY-EH6846 Size: 96T

Intended Use

The kit is intended for use in quantitative determination of Human MAPT concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Specification

Sensitivity: 18.75 pg/mL.

Detection Range: 31.2-2000 pg/mL.

No significant cross-reactivity or interference between Human MAPT and analogues was observed.

Repeatability: Coefficient of variation is < 10%.

Principle of the Procedure

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human MAPT. Standards or samples containing Human MAPT are added to the plate and reacted with capture antibody. A second anti-Human MAPT antibody labeled biotin is then added and binds to Human MAPT captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human MAPT-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human MAPT in the samples is then calculated from the OD value by establishing a standard curve.

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