Human Mab21 Domain Containing Protein 1 (MB21D1) ELISA Kit

Human Mab21 Domain Containing Protein 1 (MB21D1) ELISA Kit

Catalog No:FY-EH14332 Size: 96T

The kit isintended for use in quantitativedetermination ofHuman MB21D1 concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and otherbiological fluids.

« Sensitivity:0.1ng/mL.

« Detection Range:0.16-10ng/mL.

« No significant cross-reactivity or interference betweenHuman MB21D1 and analogues was observed.

« Repeatability:Coefficient of variation is< 4%.

Principleof the Procedure

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human MB21D1. Standards or samples containing Human MB21D1 are added to the plate and reacted with capture antibody. A second anti-Human MB21D1 antibody labeled biotinis then added and binds to Human MB21D1 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human MB21D1-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter,stopsolution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human MB21D1 in the samples is then calculated from the OD value by establishing a standard curve.

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